Acta Vet. Brno 2009, 78: 667-671
doi:10.2754/avb200978040667
ELISA for Detection of Soya Proteins in Meat Products
Eva Renčová1, Bohuslava Tremlová2
1Veterinary Research Institute, Brno, Czech Republic 2Department of Vegetable Food and Production, Faculty of Veterinary Hygiene and Ecology, Veterinary and Pharmaceutical University Brno, Czech Republic
Received July 30, 2008
Accepted June 30, 2009
Abstract
Indirect competitive ELISA method for the detection of soya proteins in meat products was developed. The detection limit of the method is 0.5% of the weight of added soya protein. A total of 131 meat product samples such as salamis or sausages from the Czech Republic market were investigated for the presence of soya proteins. Soya proteins were detected in 84% of the investigated samples without any declaration on the package of the product. The use of vegetable additives, namely soya in meat products in the market of the Czech Republic is very frequent and the restriction of its usage by legislation relates only to some kinds of durable products and ham (Act 264/2003 Coll.). The need for sensitive inspecting methods for soya protein detection is not only associated with the economic aspect (adulteration), but mainly with consumer health protection in case of allergy to soya proteins.
Keywords: Detection, immunochemical method, meat products, package declaration
Address for correspondence:
MVDr. Eva Renčová, Ph.D.Veterinary Research Institute
Department of Analytical Biotechnology
Hudcova 70, 621 00 Brno, Czech Republic
Phone: +420533331617
Fax: +420541211229
E-mail: rencova@vri.cz