Acta Vet. Brno 2009, 78: 673-678
doi:10.2754/avb200978040673
Application of DNA-based Techniques for Intraspecies Differentiation of Hake Fish
Zora Hubálková1, Petr Králík2, Janka Kasalová3, Eva Renčová1
1Department of Analytical Biotechnology, Veterinary Research Institute, Brno, Czech Republic 2Department of Food and Feed Safety, Veterinary Research Institute, Brno, Czech Republic 3Department of Meat Hygiene and Technology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
Received October 30, 2008
Accepted May 18, 2009
Abstract
Fish species identification is important as the interest of consumers in sea-fish meat is increasing. The aim of this study was to determine hake species distribution on the Czech market by the PCR-RFLP and sequencing of mt cyt b, and to develop and optimise an alternative system for determination of hake species by sequencing and/or PCR-RFLP using Pan I sequence. Among 20 samples of hake obtained on the Czech market three species were identified: North Pacific hake (Merluccius productus), Argentine hake (Merluccius hubbsi) and South Pacific hake (Merluccius gayi). The approaches tested in our study represent a significant tool either for the differentiation of hake species from other gadoid species (PCR) or for intraspecies identification of different hake species (PCR-RFLP, sequencing). This knowledge can be applied in detection of fish species substitution within the consumers' rights protection.
Keywords: Merluccius, PCR-RFLP technique, pantophysin I gene, cytochrome b gene, genomic DNA
Address for correspondence:
MVDr. Eva Renčová, Ph.D.Department of Analytical Biotechnology, Veterinary Research Institute
Hudcova 70, 621 00 Brno, Czech Republic
Phone: +420 533 331 617
Fax: +420 541 211 229
E-mail: rencova@vri.cz